12804-1-AP

antibody from Invitrogen Antibodies

Targeting: VPS26A Hbeta58, PEP8A, VPS26

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

12804-1-AP

Provider

Invitrogen Antibodies

Product name

VPS26A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LFYDGESVS GKVNLAFKQP GKRLEHQGIR IEFVGQIELF NDKSNTHEFV NLVKELALPG ELTQSRSYDF EFMQVEKPYE SYIGANVRLR YFLKVTIVRR LTDLVKEYDL IVHQLATYPD VNNSIKMEVG IEDCLHIEFE YNKSKYHLKD VIVGKIYFLL VRIKIQHMEL QLIKKEITGI GPSTTTETET IAKYEIMDGA PVKGESIPIR LFLAGYDPTP TMRDVNKKFS VRYFLNLVLV DEEDRRYFKQ QEIILWRKAP EKLRKQRTNF HQRFESPESQ ASAEQPEM (41-327 aa encoded by BC022505 )

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.21 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human kidney tissue were subjected to SDS PAGE followed by western blot with 12804-1-AP (VPS26A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12804-1-AP (VPS26A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 12804-1-AP ( VPS26A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 12804-1-AP (VPS26A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using (Product # 12804-1-AP) (VPS26A antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL555-phalloidine stains F-actin (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 12804-1-AP (VPS26A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervical cancer using 12804-1-AP (VPS26A antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 12804-1-AP ( VPS26A Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 12804-1-AP ( VPS26A Antibody) at dilution of 1:200 (under 40x lens).