Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [3]
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- Product number
- PA5-40738 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZNF217 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: SQTFTHSEDL NKHVLMQHRP TLCEPAVLRV EAEYLSPLDK SQVRTEPPKE
- Concentration
- 0.5 mg/mL
Submitted references Retracted Article: Berberine alleviates amyloid beta-induced injury in Alzheimer's disease by miR-107/ZNF217.
Wang J, Jin D
RSC advances 2019 Aug 8;9(43):25232-25239
RSC advances 2019 Aug 8;9(43):25232-25239
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human stomach cells using an anti-ZNF217 polyclonal antibody (Product # PA5-40738).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 ZNF217 is a target of miR-107. (A) The potential binding sites of miR-107 and ZNF-217. (B) Luciferase activity was measured in PC12 cells after co-transfection of ZNF217-WT or ZNF217-MUT and miR-107 mimic or miR-NC. (C) The enrichment of ZNF217 was detected in PC12 cells transfected with miR-107 mimic or miR-NC after RIP assay. (D) The protein level of ZNF217 was measured in PC12 cells transfected with miR-NC, miR-107 mimic, anti-miR-107 or anti-miR-NC by Western blot. ** P < 0.01, *** P < 0.001, ns: no significance, P > 0.05.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Fig. 5 miR-107 suppresses Abeta 25-35 -induced injury in PC12 cells by regulating ZNF217. PC12 cells were transfected with miR-NC, miR-107 mimic, miR-107 mimic and ZNF217 or pcDNA for 24 h and then treated with 1 muM berberine and 10 muM Abeta for 24 h. The ZNF217 protein level (A), expressions of IL-1beta, IL-6 and TNF-alpha in cell medium (B-D), cell apoptosis (E) and p-Tau level (F) were detected by Western blot, ELISA or flow cytometry assays, respectively. * P < 0.05, ** P < 0.01, *** P < 0.001.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Berberine decreases ZNF217 protein expression by regulating miR-107 in Abeta 25-35 -treated PC12 cells. PC12 cells were transfected with miR-NC, miR-107 mimic, anti-miR-NC or anti-miR-107 for 24 h and treated with 1 muM berberine and 10 muM Abeta for 24 h. (A and B) The abundance of ZNF217 protein was measured in treated PC12 cells by Western blot. * P < 0.05, *** P < 0.001.