LS-C407704

antibody from LSBio

Targeting: CPT1B CPT1-M, M-CPT1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C407704

Provider

LSBio

Product name

CPT1B Antibody (aa197-226) LS-C407704

Antibody type

Polyclonal

Description

Immunogen affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

CPT1B antibody IHC-paraffin. IHC(P): Mouse Skeletal Muscle Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

CPT1B antibody IHC-paraffin. IHC(P): Rat Cardiac Muscle Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC analysis of CPT1B using anti-CPT1B antibody. CPT1B was detected in frozen section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CPT1B Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC analysis of CPT1B using anti-CPT1B antibody. CPT1B was detected in frozen section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CPT1B Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

CPT1B antibody IHC-paraffin. IHC(P): Mouse Skeletal Muscle Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

CPT1B antibody IHC-paraffin. IHC(P): Rat Cardiac Muscle Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC analysis of CPT1B using anti-CPT1B antibody. CPT1B was detected in frozen section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CPT1B Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC analysis of CPT1B using anti-CPT1B antibody. CPT1B was detected in frozen section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CPT1B Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

CPT1B antibody IHC-paraffin. IHC(P): Mouse Skeletal Muscle Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

CPT1B antibody IHC-paraffin. IHC(P): Rat Cardiac Muscle Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

CPT1B antibody IHC-paraffin. IHC(P): Mouse Skeletal Muscle Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

CPT1B antibody IHC-paraffin. IHC(P): Rat Cardiac Muscle Tissue.