MA5-34745

antibody from Invitrogen Antibodies

Targeting: UQCRFS1 RIP1, RIS1, RISP, UQCR5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34745

Provider

Invitrogen Antibodies

Product name

UQCRFS1 Recombinant Rabbit Monoclonal Antibody (JG62-31)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: A549, 293, mouse kidney tissue lysate, 293T, LOVO, SiHa, rat skeletal muscle tissue, human colon cancer tissue, human appendix tissue, human kidney tissue, mouse cerebellum tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JG62-31

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of UQCRFS1 in Lane 1: A549, Lane 2: 293, Lane 3: Mouse kidney. Samples were incubated with UQCRFS1 monoclonal antibody (Product # MA5-34745), at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of UQCRFS1 in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with UQCRFS1 monoclonal antibody (Product # MA5-34745), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of UQCRFS1 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with UQCRFS1 monoclonal antibody (Product # MA5-34745), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of UQCRFS1 in 293T cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with UQCRFS1 monoclonal antibody (Product # MA5-34745), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of UQCRFS1 in paraffin-embedded rat skeletal muscle tissue. Samples were incubated with UQCRFS1 monoclonal antibody (Product # MA5-34745), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of UQCRFS1 in paraffin-embedded mouse cerebellum tissue. Samples were incubated with UQCRFS1 monoclonal antibody (Product # MA5-34745), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of UQCRFS1 in paraffin-embedded human kidney tissue. Samples were incubated with UQCRFS1 monoclonal antibody (Product # MA5-34745), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of UQCRFS1 in paraffin-embedded human colon cancer tissue. Samples were incubated with UQCRFS1 monoclonal antibody (Product # MA5-34745), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of UQCRFS1 in paraffin-embedded human appendix tissue. Samples were incubated with UQCRFS1 monoclonal antibody (Product # MA5-34745), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of UQCRFS1 in A549 cells (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple). Samples were incubated with UQCRFS1 monoclonal antibody (Product # MA5-34745) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.