Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-B434 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B434, RRID:AB_2241491
- Product name
- IHC-plus™ USP7 / HAUSP Antibody LS-B434
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: -20°C; Long term: -20°C.
No comments: Submit comment
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-HAUSP Antibody - Western Blot. Western blot of affinity purified anti-HAUSP antibody shows detection of HAUSP in various cell lysates at 130 kD (lane 1 - HeLa nuclear extract, lane 2 - HeLa, Lane 3 - A431, Lane 4 - MCF7, Lane 5 - 3T3). The antibody is blocked by pre-incubation with the immunizing peptide (lanes 6 - 10) using the same lysates. A 1:500 dilution of the primary antibody was used for detection followed by a 1:5000 dilution of HRP Gt-a-Rabbit IgG. Exposure time was 30 s.