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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA1-31831 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- USP7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-31831 detects HAUSP from human samples.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1.72 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of HAUSP in HeLa nuclear extract (Lane 1) HeLa (Lane 2) A431 (Lane 3) MCF7 (Lane 4) and NIH-3T3 (Lane 5) and blocked by pre-incubation with the immunizing peptide (lanes 6 - 10) using the same lysates using a HAUSP polyclonal antibody (Product # PA1-31831) at a dilution of 1:500 and detected using a 1:5000 dilution of HRP Goat-anti-Rabbit IgG at an Exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot using USP7 Polyclonal Antibody (Product # PA1-31831) shows detection of HAUSP in various cell lysates at 130 kDa (lane 1 - HeLa nuclear extract, lane 2 - HeLa, Lane 3 - A431, Lane 4 - MCF7, Lane 5 - 3T3). The antibody is blocked by pre-incubation with the immunizing peptide (lanes 6 - 10) using the same lysates. A 1:500 dilution of the primary antibody was used for detection followed by a 1:5,000 dilution of HRP Gt-a-Rabbit IgG. Exposure time was 30 s.
