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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB10225 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10225, RRID:AB_1716860
- Product name
- USP7 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of USP7.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Molecular recognition of p53 and MDM2 by USP7/HAUSP.
Reciprocal activities between herpes simplex virus type 1 regulatory protein ICP0, a ubiquitin E3 ligase, and ubiquitin-specific protease USP7.
Expression and functional analyses of mHAUSP regulating apoptosis of cervical adenocarcinoma cells.
Sheng Y, Saridakis V, Sarkari F, Duan S, Wu T, Arrowsmith CH, Frappier L
Nature structural & molecular biology 2006 Mar;13(3):285-91
Nature structural & molecular biology 2006 Mar;13(3):285-91
Reciprocal activities between herpes simplex virus type 1 regulatory protein ICP0, a ubiquitin E3 ligase, and ubiquitin-specific protease USP7.
Boutell C, Canning M, Orr A, Everett RD
Journal of virology 2005 Oct;79(19):12342-54
Journal of virology 2005 Oct;79(19):12342-54
Expression and functional analyses of mHAUSP regulating apoptosis of cervical adenocarcinoma cells.
Yoo KJ, Lee HJ, Lee H, Lee KY, Lee SH, Chung HM, Baek KH
International journal of oncology 2005 Jul;27(1):97-104
International journal of oncology 2005 Jul;27(1):97-104
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using USP7 polyclonal antibody (Cat # PAB10225) shows detection of USP7 in various cell lysates at 130 kDa (lane 1 - HeLa nuclear extract, lane 2 - HeLa, Lane 3 - A-431, Lane 4 - MCF-7, Lane 5 - 3T3).The antibody is blocked by pre-incubation with the immunizing peptide (lanes 6 - 10) using the same lysates.A 1:500 dilution of the primary antibody was used for detection followed by a 1:5,000 dilution of HRP Gt-a-Rabbit IgG.Exposure time was 30 sec.
