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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [7]
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- Product number
- 20449-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KRT34 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: WFCEGSFNG SEKETMQFLN DRLASYLEKV RQLERDNAEL EKLIQERSQQ QEPLLCPSYQ SYFKTIEELQ QKILCAKAEN ARLVVNIDNA KLASDDFRSK YQTEQSLRLL VESDINSIRR ILDELTLCKS DLESQVESLR EELICLKKNH EEEVNTLRSQ LGDRLNVEVD TAPTVDLNQV LNETRSQYEA LVETNRREVE QWFATQTEEL NKQVVSSSEQ LQSCQAEIIE LRRTVNALEI ELQAQHNLRD SLENTLTESE AHYSSQLSQV QSLITNVESQ LAEIRCDLER QNQEYQVLLD VRARLECEIN TYRSLLESED CKLPCNPCAT TNASGNSCGP CGTSQKGCCN (88-436 aa encoded by BC041070)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.54 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 20449-1-AP ( KRT34 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- L02 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A375 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 20449-1-AP (KRT34 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 20449-1-AP (KRT34 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 40x lens).
