HPA042182

antibody from Atlas Antibodies

Targeting: CIAPIN1 Anamorsin, CIAE2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA042182

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA042182, RRID:AB_10794646

Product name

Anti-CIAPIN1

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

KLWTLSANDMEDDSMDLIDSDELLDPEDLKKPDPA
SLRAASCGEGKKRKACKNCTCGLAEELEKEKSREQ
MSSQPKSACGNCYLGDAFRCASCPYLGMPAFKPGE
KVLLSD


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Submitted references

The Diabetes Drug Target MitoNEET Governs a Novel Trafficking Pathway to Rebuild an Fe-S Cluster into Cytosolic Aconitase/Iron Regulatory Protein 1

Ferecatu I, Goncalves S, Golinelli-Cohen M, Clemancey M, Martelli A, Riquier S, Guittet E, Latour J, Puccio H, Drapier J, Lescop E, Bouton C
Journal of Biological Chemistry 2014 October;289(41):28070-28086

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human testis and skeletal muscle tissues using Anti-CIAPIN1 antibody. Corresponding CIAPIN1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Show more

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows low expression as expected.

Sample type

HUMAN