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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- TA500346 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500346, RRID:AB_2235777
- Product name
- Anti-NEUROG3 (Neurogenin3) mouse monoclonal antibody, clone OTI3B5 (formerly 3B5)
- Antibody type
- Monoclonal
- Description
- Anti-NEUROG3 (Neurogenin3) mouse monoclonal antibody, clone OTI3B5 (formerly 3B5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NEUROG3
- Isotype
- IgG
- Antibody clone number
- OTI3B5
- Vial size
- 100 µl
- Concentration
- 1.3 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEUROG3 (RC224767, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEUROG3.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-NEUROG3 mouse monoclonal antibody (NEUROG3) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY NEUROG3 (RC224767).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation of NEUROG3 by using TrueMab monoclonal anti-NEUROG3 antibody (Negative control: IP without adding anti-NEUROG3 antibody). For each experiment, 500ul of DDK tagged NEUROG3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2u
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-NEUROG3 mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NEUROG3 mouse monoclonal antibody (TA500346) at 1:150 dilution.
- Validation comment
- IHC
