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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- MA5-24911 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NeuroG3 Monoclonal Antibody (OTI3B5)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI3B5
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NEUROG3 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with NEUROG3 (Product # MA5-24911) monoclonal antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NEUROG3 in COS7 cells. Cells were transfected with a plasmid overexpressing NEUROG3 and probed with a NEUROG3 monoclonal antibody (Product # MA5-24911).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NEUROG3 in paraffin-embedded human liver tissue using a NEUROG3 monoclonal antibody (Product # MA5-24911) at a dilution of 1:150.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human lymph node tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a NEUROG3 monoclonal antibody (Product # MA5-24911) at a dilution of 1:150.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of NEUROG3 in HEK293T cells. The cells were incubated with monoclonal NEUROG3 (Product # MA5-24911) antibody using 2 µg of sample. Lanes 1-2 contain cell lysate incubated with &B3& antibody derived from different clones.
