15479-1-AP

antibody from Invitrogen Antibodies

Targeting: UNC45A GC-UNC45, SMAP-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15479-1-AP

Provider

Invitrogen Antibodies

Product name

UNC45A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: EMTFPGERI YEVVRPLVSL LHLNCSGLQN FEALMALTNL AGISERLRQK ILKEKAVPMI EGYMFEEHEM IRRAATECMC NLAMSKEVQD LFEAQGNDRL KLLVLYSGED DELLQRAAAG GLAMLTSMRP TLCSRIPQVT THWLEILQAL LLSSNQELQH RGAVVVLNMV EASREIASTL MESEMMEILS VLAKGDHSPV TRAAAACLDK AVEYGLIQPN QDGE (722-944 aa encoded by B C006214)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.17 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 15479-1-AP (UNC45A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of UNC45A antibody (15479-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UNC45A transfected HeLa cells.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using UNC45A antibody 15479-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using UNC45A antibody 15479-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervix using 15479-1-AP (UNC45A antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervix using 15479-1-AP (UNC45A antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-UNC45A (IP:15479-1-AP, 4ug; Detection:15479-1-AP 1:1000) with HeLa cells lysate 1400ug.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-UNC45A (IP:15479-1-AP, 4 ug; Detection:15479-1-AP 1:300) with HeLa cells lysate 1600 ug.