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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Flow cytometry [1]
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- Product number
- MAB1364 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CC Chemokine Receptor D6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CC Chemokine Receptor D6 in transfectants. Does not stain the parent cell line.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
O00590- Isotype
- IgG
- Antibody clone number
- 196124
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The N-terminal region of the atypical chemokine receptor ACKR2 is a key determinant of ligand binding.
Recognition versus adaptive up-regulation and degradation of CC chemokines by the chemokine decoy receptor D6 are determined by their N-terminal sequence.
Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation.
Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6.
Hewit KD, Fraser A, Nibbs RJ, Graham GJ
The Journal of biological chemistry 2014 May 2;289(18):12330-42
The Journal of biological chemistry 2014 May 2;289(18):12330-42
Recognition versus adaptive up-regulation and degradation of CC chemokines by the chemokine decoy receptor D6 are determined by their N-terminal sequence.
Savino B, Borroni EM, Torres NM, Proost P, Struyf S, Mortier A, Mantovani A, Locati M, Bonecchi R
The Journal of biological chemistry 2009 Sep 18;284(38):26207-15
The Journal of biological chemistry 2009 Sep 18;284(38):26207-15
Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation.
Bonecchi R, Borroni EM, Anselmo A, Doni A, Savino B, Mirolo M, Fabbri M, Jala VR, Haribabu B, Mantovani A, Locati M
Blood 2008 Aug 1;112(3):493-503
Blood 2008 Aug 1;112(3):493-503
Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6.
Martinez de la Torre Y, Buracchi C, Borroni EM, Dupor J, Bonecchi R, Nebuloni M, Pasqualini F, Doni A, Lauri E, Agostinis C, Bulla R, Cook DN, Haribabu B, Meroni P, Rukavina D, Vago L, Tedesco F, Vecchi A, Lira SA, Locati M, Mantovani A
Proceedings of the National Academy of Sciences of the United States of America 2007 Feb 13;104(7):2319-24
Proceedings of the National Academy of Sciences of the United States of America 2007 Feb 13;104(7):2319-24
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CC Chemokine Receptor D6 in NS0 Mouse Cell Line Transfected with Human CC Chemokine Receptor D6 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human CC Chemokine Receptor D6 was stained with Rat Anti-Human CC Chemokine Receptor D6 Monoclonal Antibody (Catalog # MAB1364, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
