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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA504217 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504217, RRID:AB_11141765
- Product name
- ALG2 mouse monoclonal antibody, clone OTI2A3 (formerly 2A3)
- Antibody type
- Monoclonal
- Description
- ALG2 mouse monoclonal antibody, clone OTI2A3 (formerly 2A3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ALG2
- Isotype
- IgG
- Antibody clone number
- OTI2A3
- Vial size
- 100 µl
- Concentration
- 0.83 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALG2 (RC204766, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALG2.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-ALG2 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-ALG2 mouse monoclonal antibody (TA504217) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALG2(RC204766).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ALG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504217)
- Validation comment
- IHC
