PA5-53704

antibody from Invitrogen Antibodies

Targeting: CCDC186 C10orf118, CCCP-1, FLJ10188, FLJ35301, golgin104

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-53704

Provider

Invitrogen Antibodies

Product name

C10orf118 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LKRTFKEGMD ELRTLRTKVK CLEDERLRTE DELSKYKEII NRQKAEIQNL LDKVKTADQL QEQLQRGKQE IENLKEEVES LNSLINDLQK DIEGSRKRES ELLLFTERLT SKNAQLQSES NSLQSQFDKV SC

Concentration

0.3 mg/mL

References

Submitted references

The Secreted Protein C10orf118 Is a New Regulator of Hyaluronan Synthesis Involved in Tumour-Stroma Cross-Talk.

Caon I, D'Angelo ML, Bartolini B, Caravà E, Parnigoni A, Contino F, Cancemi P, Moretto P, Karamanos NK, Passi A, Vigetti D, Karousou E, Viola M
Cancers 2021 Mar 5;13(5)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of C10orf118 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4. Samples were probed using a C10orf118 Polyclonal Antibody (Product # PA5-53704).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of C10orf118 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a C10orf118 Polyclonal Antibody (Product # PA5-53704).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of C10orf118 in human cell line A-431 shows positivity in the Golgi apparatus. Samples were probed using a C10orf118 Polyclonal Antibody (Product # PA5-53704).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of C10orf118 in human duodenum tissue shows strong cytoplasmic positivity in glandular cells. Samples were probed using a C10orf118 Polyclonal Antibody (Product # PA5-53704).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Endogenous c10orf118 localizes in the Golgi apparatus in MCF-7 cells. ( A ) Confocal microscopy images of MCF-7 cells stained for c10orf118 (green) and Golgin-97 (red), a Golgi apparatus marker. ( B ) Confocal microscopy images of MCF-7 cells stained for c10orf118 (green) and calnexin (red), a specific endoplasmic reticulum marker. ( C ) Line-scan of the staining performed in the section marked with a green line in the merged view and relative quantification. Overlap of the two signals is apparent. ( D ) Line-scan of the staining in the section marked with a green line in the merged view and relative quantification. ( E ) Confocal microscopy images of MCF-7 cells nucleofected with 30 nmol of scrambled siRNA and siRNA against c10orf118 for 48 h and stained with antibody against c10orf118 (green) or Golgin-97 (red). White bars, 20 um.