PA5-58017

antibody from Invitrogen Antibodies

Targeting: PCBD1 DCOH, PCBD, PCD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-58017

Provider

Invitrogen Antibodies

Product name

PCBD1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MAGKAHRLSA EERDQLLPNL RAVGWNELEG RDAIFKQFHF KDFNRAFGFM TR

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-PCBD1 Polyclonal Antibody (Product # PA5-58017) and a 12 kDa band corresponding to Pterin-4-alpha-carbinolamine dehydratase was observed across cell lines and tissue extracts tested and not in MOLT-4, Reh, Mouse Spleen and Mouse Brain. Whole cell extracts (30 µg lysate) of Caco-2 (Lane 1), Hep G2 (Lane 2), A549 (Lane 3), U-2 OS (Lane 4), MOLT-4 (Lane 5), Reh (Lane 6) and tissue extracts of Mouse Spleen (Lane 7), Mouse Brain (Lane 8), Mouse Kidney (Lane 9), Mouse Liver (Lane 10) and Rat Liver (Lane 11) were electrophoresed using Novex™ 16% Tricine Protein Gel (Product # EC66952BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of PCBD1 in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a PCBD1 Polyclonal Antibody (Product # PA5-58017).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Pterin-4-alpha-carbinolamine dehydratase was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PCBD1 Polyclonal Antibody (Product # PA5-58017) at 2 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing predominantly nuclear and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PCBD1 in human rectal tissue shows strong cytoplasmic positivity in endocrine cells. Samples were probed using a PCBD1 Polyclonal Antibody (Product # PA5-58017).