H00056254-B01

antibody from Abnova Corporation

Targeting: RNF20 BRE1, BRE1A, FLJ11189, FLJ20382, hBRE1, KAIA2779

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00056254-B01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00056254-B01, RRID:AB_11189691

Product name

RNF20 MaxPab mouse polyclonal antibody (B01)

Antibody type

Polyclonal

Description

Mouse polyclonal antibody raised against a full-length human RNF20 protein.

Antigen sequence

MSGIGNKRAAGEPGTSMPPEKKAAVEDSGTTVETI
KLGGVSSTEELDIRTLQTKNRKLAEMLDQRQAIED
ELREHIEKLERRQATDDASLLIVNRYWSQFDENIR
IILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQ
ERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQ
ERVESSRRAVSQIVTVYDKLQEKVELLSRKLNSGD
NLIVEEAVQELNSFLAQENMRLQELTDLLQEKHRT
MSQEFSKLQSKVETAESRVSVLESMIDDLQWDIDK
IRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYG
GTITINARKFEEMNAELEENKELAQNRLCELEKLR
QDFEEVTTQNEKLKVELRSAVEQVVKETPEYRCMQ
SQFSVLYNESLQLKAHLDEARTLLHGTRGTHQHQV
ELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEML
RIEFEQTLAANEQAGPINREMRHLISSLQNHNHQL
KGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSS
TEDPKDEPAELKPDSEDLSSQSSASKASQEDANEI
KSKRDEEERERERREKEREREREREKEKEREREKQ
KLKESEKERDSAKDKEKGKHDDGRKKEAEIIKQLK
IELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMA
AEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDA
LRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVT
GQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKS
NQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKL
EEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEA
AQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKD
MFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILM
EEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFEC
VKTRYDTRQRKCPKCNAAFGANDFHRIYIG


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RNF20 expression in transfected 293T cell line (H00056254-T01) by RNF20 MaxPab polyclonal antibody.Lane 1: RNF20 transfected lysate(107.25 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

RNF20 MaxPab polyclonal antibody. Western Blot analysis of RNF20 expression in HL-60.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

RNF20 MaxPab polyclonal antibody. Western Blot analysis of RNF20 expression in HeLa.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to RNF20 on HeLa cell. [antibody concentration 20 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol