12184-1-AP

antibody from Invitrogen Antibodies

Targeting: NDRG4 KIAA1180, SMAP-8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

12184-1-AP

Provider

Invitrogen Antibodies

Product name

NDRG4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MPECWDGEH DIETPYGLLH VVIRGSPKGN RPAILTYHDV GLNHKLCFNT FFNFEDMQEI TKHFVVCHVD APGQQVGASQ FPQGYQFPSM EQLAAMLPSV VQHFGFKYVI GIGVGAGAYV LAKFALIFPD LVEGLVLVNI DPNGKGWIDW AATKLSGLTS TLPDTVLSHL FSQEELVNNT ELVQSYRQQI GNVVNQANLQ LFWNMYNSRR DLDINRPGTV PNAKTLRCPV MLVVGDNAPA EDGVVECNSK LDPTTTTFLK MADSGGLPQV TQPGKLTEAF KYFLQGMGYM PSASMTRLAR SRTASLTSAS SVDGSRPQAC THSESSEGLG QVNHTMEVSC (1-339 aa encoded by BC011795)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 12184-1-AP (NDRG4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12184-1-AP (NDRG4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using NDRG4 antibody 12184-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using NDRG4 antibody 12184-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon tissue slide using 12184-1-AP (NDRG4 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon tissue slide using 12184-1-AP (NDRG4 Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12184-1-AP (NDRG4 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12184-1-AP (NDRG4 Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human medulloblastoma tissue slide using 12184-1-AP ( NDRG4 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human medulloblastoma tissue slide using 12184-1-AP ( NDRG4 Antibody) at dilution of 1:200 (under 40x lens).