MA1-22740

antibody from Invitrogen Antibodies

Targeting: MB PVALB

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

MA1-22740

Provider

Invitrogen Antibodies

Product name

Myoglobin Monoclonal Antibody (4E2)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-22740 detects Myoglobin in Human samples.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4E2

Vial size

100 µg

Concentration

2 mg/mL

Storage

4° C, do not freeze

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Myoglobin Monoclonal Antibody (4E2) (Product # MA1-22740) and a 17 kDa band corresponding to Myoglobin was observed only in Mouse and Rat, Heart and Skeletal Muscle and not in any other tissues. Tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2), Mouse Skeletal Muscle (Lane 3), Rat Skeletal Muscle (Lane 4), Mouse Liver (Lane 5), Rat Liver (Lane 6), Mouse Kidney (Lane 7), Mouse Lung (Lane 8) and Mouse Adipose (Lane 9) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).