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Learn10751-1-AP
antibody from Invitrogen Antibodies
Targeting: NIPSNAP3A
DKFZp564D177, FLJ13953, HSPC299, MGC14553
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 10751-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NIPSNAP3A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MLVLRSALT RALASRTLAP QMCSSFATGP RQYDGIFYEF RSYYLKPSKM NEFLENFEKN AHLRTAHSEL VGYWSVEFGG RMNTVFHIWK YDNFAHRTEV RKALAKDKEW QEQFLIPNLA LIDKQESEIT YLVPWCKLEK PPKEGVYELA TFQMKPGGPA LWGDAFKRAV HAHVNLGYTK LVGVFHTEYG ALNRVHVLWW NESADSRAAG RHKSHEDPRV VAAVRESVNY LVSQQNMLLI PTSFSPLK (1-247 aa encoded by BC005935)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 10751-1-AP (NIPSNAP3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 10751-1-AP (NIPSNAP3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10751-1-AP (NIPSNAP3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HEK-293 cells using 10751-1-AP (NIPSNAP3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10751-1-AP ( NIPSNAP3A Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10751-1-AP ( NIPSNAP3A Antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-NIPSNAP3A (IP:10751-1-AP, 4ug; Detection:10751-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
