13162-1-AP

antibody from Invitrogen Antibodies

Targeting: RASGRP3 CalDAG-GEFIII, GRP3, KIAA0846

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

13162-1-AP

Provider

Invitrogen Antibodies

Product name

RASGRP3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: SLQNASHHL EPNMDLINLL TLSLDLYHTE DDIYKLSLVL EPRNSKSQPT SPTTPNKPVV PLEWALGVMP KPDPTVINKH IRKLVESVFR NYDHDHDGYI SQEDFESIAA NFPFLDSFCV LDKDQDGLIS KDEMMAYFLR AKSQLHCKMG PGFIHNFQEM TYLKPTFCEH CAGFLWGIIK QGYKCKDCGA NCHKQCKDLL VLACRRFARA PSLSSGHGSL PGSPSLPPAQ DEVFEFPGVT AGHRDLDSRA ITLVTGSSRK ISVRLQRATT SQATQTEPVW SEAGWGDSGS HTFPKMKSKF HDKAAKDKGF AKWENEKPRV HAGVDVVDRG TEFELDQDEG EETRQDGEDG (342-690 aa encoded by BC027849)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13162-1-AP (RASGRP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 13162-1-AP (RASGRP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Ramos cells were subjected to SDS PAGE followed by western blot with 13162-1-AP (RASGRP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tumor slide using 13162-1-AP (RASGRP3 Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tumor slide using 13162-1-AP (RASGRP3 Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tumor slide using 13162-1-AP ( RASGRP3 Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tumor slide using 13162-1-AP ( RASGRP3 Antibody) at dilution of 1:50.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RASGRP3 (IP: 13162-1-AP, 4 µg; Detection: 13162-1-AP 1:500) with RAW 264.7 cells lysate 3200 µg.