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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [3]
- Immunohistochemistry [5]
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- Product number
- NBP1-86663 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86663, RRID:AB_11021790
- Product name
- Rabbit Polyclonal CBARA1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human CBARA1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references A High-Throughput Screening Identifies MICU1 Targeting Compounds.
MICU1 Alleviates Diabetic Cardiomyopathy Through Mitochondrial Ca2+-Dependent Antioxidant Response.
Structure and function of the N-terminal domain of the human mitochondrial calcium uniporter.
Mitofusin-2 triggers mitochondria Ca2+ influx from the endoplasmic reticulum to induce apoptosis in hepatocellular carcinoma cells.
Directed evolution of APEX2 for electron microscopy and proximity labeling.
Loss-of-function mutations in MICU1 cause a brain and muscle disorder linked to primary alterations in mitochondrial calcium signaling.
Di Marco G, Vallese F, Jourde B, Bergsdorf C, Sturlese M, De Mario A, Techer-Etienne V, Haasen D, Oberhauser B, Schleeger S, Minetti G, Moro S, Rizzuto R, De Stefani D, Fornaro M, Mammucari C
Cell reports 2020 Feb 18;30(7):2321-2331.e6
Cell reports 2020 Feb 18;30(7):2321-2331.e6
MICU1 Alleviates Diabetic Cardiomyopathy Through Mitochondrial Ca2+-Dependent Antioxidant Response.
Ji L, Liu F, Jing Z, Huang Q, Zhao Y, Cao H, Li J, Yin C, Xing J, Li F
Diabetes 2017 Jun;66(6):1586-1600
Diabetes 2017 Jun;66(6):1586-1600
Structure and function of the N-terminal domain of the human mitochondrial calcium uniporter.
Lee Y, Min CK, Kim TG, Song HK, Lim Y, Kim D, Shin K, Kang M, Kang JY, Youn HS, Lee JG, An JY, Park KR, Lim JJ, Kim JH, Kim JH, Park ZY, Kim YS, Wang J, Kim DH, Eom SH
EMBO reports 2015 Oct;16(10):1318-33
EMBO reports 2015 Oct;16(10):1318-33
Mitofusin-2 triggers mitochondria Ca2+ influx from the endoplasmic reticulum to induce apoptosis in hepatocellular carcinoma cells.
Wang W, Xie Q, Zhou X, Yao J, Zhu X, Huang P, Zhang L, Wei J, Xie H, Zhou L, Zheng S
Cancer letters 2015 Mar 1;358(1):47-58
Cancer letters 2015 Mar 1;358(1):47-58
Directed evolution of APEX2 for electron microscopy and proximity labeling.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY
Nature methods 2015 Jan;12(1):51-4
Nature methods 2015 Jan;12(1):51-4
Loss-of-function mutations in MICU1 cause a brain and muscle disorder linked to primary alterations in mitochondrial calcium signaling.
Logan CV, Szabadkai G, Sharpe JA, Parry DA, Torelli S, Childs AM, Kriek M, Phadke R, Johnson CA, Roberts NY, Bonthron DT, Pysden KA, Whyte T, Munteanu I, Foley AR, Wheway G, Szymanska K, Natarajan S, Abdelhamed ZA, Morgan JE, Roper H, Santen GW, Niks EH, van der Pol WL, Lindhout D, Raffaello A, De Stefani D, den Dunnen JT, Sun Y, Ginjaar I, Sewry CA, Hurles M, Rizzuto R, UK10K Consortium., Duchen MR, Muntoni F, Sheridan E
Nature genetics 2014 Feb;46(2):188-93
Nature genetics 2014 Feb;46(2):188-93
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: CBARA1 Antibody [NBP1-86663] - Simple Western lane view shows a specific band for CBARA1 in 0.2 mg/ml of RT-4 (left), U-251 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: CBARA1 Antibody [NBP1-86663] - Electropherogram image(s) of corresponding Simple Western lane view. CBARA1 antibody was used at 1:20 dilution on RT-4 and U-251 lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CBARA1 Antibody [NBP1-86663] - Analysis in human cell line A-549.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CBARA1 Antibody [NBP1-86663] - Staining of human adrenal gland shows strong cytoplasmic and nuclear positivity in cortical cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CBARA1 Antibody [NBP1-86663] - Staining of human kidney shows strong granular positivity in cytoplasm in cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CBARA1 Antibody [NBP1-86663] - Staining of human endometrium shows strong granular positivity in cytoplasm in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CBARA1 Antibody [NBP1-86663] - Staining of human rectum shows strong granular positivity in cytoplasm in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CBARA1 Antibody [NBP1-86663] - Staining of human liver shows moderate granular positivity in cytoplasm in hepatocytes.
