AP7273b

antibody from Abgent

Targeting: NAE1 APP-BP1, APPBP1, ula-1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7273b

Provider

Abgent

Proper citation

Abgent Cat#AP7273b, RRID:AB_1208408

Product name

NAE1 (APPBP1) Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of anti-APPBP1 Antibody (C-term) (Cat.#AP7273b) in mouse brain tissue lysates (35ug/lane). APPBP1(arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of APP-BP1(arrow) using rabbit polyclonal APP-BP1 Antibody (C-term) (Cat.#AP7273b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APP-BP1 gene (Lane 2) (Origene Technologies).

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human breast carcinoma reacted with anti-APPBP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:50~100