PA5-59836

antibody from Invitrogen Antibodies

Targeting: NAE1 APP-BP1, APPBP1, ula-1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-59836

Provider

Invitrogen Antibodies

Product name

NAE1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: KPFPELREHF QSYDLDHMEK KDHSHTPWIV IIAKYLAQWY SETNGRIPKT YKEKEDFRDL IRQGILKNEN GAPEDEENFE EAIKNVNTAL NTTQIPSSIE DIFNDDR

Concentration

0.2 mg/mL

References

Submitted references

Organoid-based drug screening reveals neddylation as therapeutic target for malignant rhabdoid tumors.

Calandrini C, van Hooff SR, Paassen I, Ayyildiz D, Derakhshan S, Dolman MEM, Langenberg KPS, van de Ven M, de Heus C, Liv N, Kool M, de Krijger RR, Tytgat GAM, van den Heuvel-Eibrink MM, Molenaar JJ, Drost J
Cell reports 2021 Aug 24;36(8):109568

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NAE1 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a NAE1 Polyclonal Antibody (Product # PA5-59836).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Nae1 was performed by loading whole cell lysate in 1X LDS sample buffer with 2-ME from 5 x 10^5 mouse transformed pre-B cells. Samples were loaded onto a 4-12 % Bis-Tris polyacrylamide gel (Product # NP0336BOX). Proteins were transferred to nitrocellulose membrane with wet/tank transfer. Membrane was blocked in 5% milk/TBST. The membrane was probe with a polyclonal anti-Nae1 antibody (Product # PA5-59836) at a dilution of 1:500 in 5% milk/TBST at 4oC overnight, followed by a secondary antibody HRP-anti-rabbit at a dilution of 1:10000 at room temperature for 1 hour. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # PI-32209). Data courtesy of Thermo Scientific KOL Program.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NAE1 using NAE1 Polyclonal Antibody (Product # PA5-59836) (A) shows similar pattern to an independent NAE1 Polyclonal Antibody (B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NAE1 in human cerebral cortex using NAE1 Polyclonal Antibody (Product # PA5-59836).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NAE1 in human testis using NAE1 Polyclonal Antibody (Product # PA5-59836).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NAE1 in human liver using NAE1 Polyclonal Antibody (Product # PA5-59836).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NAE1 in human colon using NAE1 Polyclonal Antibody (Product # PA5-59836).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NAE1 in human cerebellum using a NAE1 Polyclonal Antibody (Product # PA5-59836) shows strong nuclear positivity in Purkinje cells.