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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
- Immunohistochemistry [9]
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- Product number
- NBP1-92162 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-92162, RRID:AB_11016793
- Product name
- Rabbit Polyclonal NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat NEDD8 Activating Enzyme (APPBP1/UBA3) antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references PPARĪ³ neddylation essential for adipogenesis is a potential target for treating obesity.
Park HS, Ju UI, Park JW, Song JY, Shin DH, Lee KH, Jeong LS, Yu J, Lee HW, Cho JY, Kim SY, Kim SW, Kim JB, Park KS, Chun YS
Cell death and differentiation 2016 Aug;23(8):1296-311
Cell death and differentiation 2016 Aug;23(8):1296-311
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody [NBP1-92162] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody [NBP1-92162] - Analysis using Anti-NAE1 antibody NBP1-92162 (A) shows similar pattern to independent antibody NBP1-92163 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody [NBP1-92162] - Staining of human cerebral cortex.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody [NBP1-92162] - Staining of human colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody [NBP1-92162] - Staining of human testis.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody [NBP1-92162] - Staining of human liver.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody [NBP1-92162] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody [NBP1-92162] - Staining of human cerebral cortex, colon, prostate and testis using Anti-NAE1 antibody NBP1-92162 (A) shows similar protein distribution across tissues to independent antibody NBP1-92163 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody [NBP1-92162] - Staining of human colon shows strong nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody [NBP1-92162] - Staining of human prostate shows strong nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody [NBP1-92162] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
