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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- PA5-89033 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: AFKVPRPGPD TLQFTVDVFH FANDSRNMIY ITCHLKVTLA EQDPDELNKA CSFSKPSNSW FPVEGSADIC QCCNKGDCGT PSHSRRQPHV MSQWSRSASR N; Positive Samples: HeLa; Cellular Location: Cell membrane, Single-pass type I membrane protein, extracellular matrix, extracellular space
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.53 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of HeLa cells, using ZP3 Polyclonal antibody (Product # PA5-89033) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ZP3 in extracts of HeLa cells using ZP3 Polyclonal Antibody (Product # PA5-89033) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry-Immunofluorescence analysis of ZP3 was performed in rat oophoroma tissue using ZP3 Polyclonal Antibody (Product # PA5-89033) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry-Immunofluorescence analysis of ZP3 was performed in mouse oophoroma tissue using ZP3 Polyclonal Antibody (Product # PA5-89033) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry-Immunofluorescence analysis of ZP3 was performed in mouse oophoroma tissue using ZP3 Polyclonal Antibody (Product # PA5-89033) at a dilution of 1:100. Blue: DAPI for nuclear staining.
