21279-1-AP

antibody from Invitrogen Antibodies

Targeting: ZP3 ZP3-372, ZP3-424, ZP3A, ZP3B, ZPC

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

21279-1-AP

Provider

Invitrogen Antibodies

Product name

ZP3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MVMVSKDLF GTGKLIRAAD LTLGPEACEP LVSMDTEDVV RFEVGLHECG NSMQVTDDAL VYSTFLLHDP RPVGNLSIVR TNRAEIPIEC RYPRQGNVSS QAILPTWLPF RTTVFSEEKL TFSLRLMEEN WNAEKRSPTF HLGDAAHLQA EIHTGSHVPL RLFVDHCVAT PTPDQNASPY HTIVDFHGCL VDGLTDASSA F (1-200 aa encoded by BC113949)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of 21279-1-AP (ZP3) from Dr. Soory. (Target band 41-47kd,56kd (Glycosylated form, PMID:19700799),83kd (PMID: 1577197).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA) fixed mouse ovary tissue using 21279-1-AP (ZP3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA) fixed mouse ovary tissue using 21279-1-AP (ZP3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA) fixed mouse ovary tissue using 21279-1-AP (ZP3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse ovary tissue slide using 21279-1-AP (ZP3 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Sodium Citrate buffer (pH6).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse ovary tissue slide using 21279-1-AP (ZP3 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Sodium Citrate buffer (pH6).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ZP3 (IP:21279-1-AP, 4ug; Detection:21279-1-AP 1:500) with HeLa cells lysate 1600ug.