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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
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- Product number
- PA5-22167 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-SNRK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, H1299, HCT116, H1299 nuclear extract. Predicted reactivity: Mouse (95%), Rat (96%), Chicken (89%), Rhesus Monkey (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.52 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SNRK using 30 µg of H1299 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SNRK polyclonal antibody (Product # PA5-22167) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SNRK in H1299 whole cell extracts and nuclear extracts (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with SNRK, Internal Polyclonal antibody (Product # PA5-22167) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SNRK in Various whole cell extracts (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with SNRK, Internal Polyclonal antibody (Product # PA5-22167) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SNRK was performed by separating 30 µg of H1299 whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a SNRK Polyclonal Antibody (Product # PA5-22167) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SNRK was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SNRK Polyclonal Antibody (Product # PA5-22167) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
