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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Chromatin Immunoprecipitation [1]
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- Product number
- ABIN2616387 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Ankyrin Repeat and Sterile alpha Motif Domain Containing 1A (ANKS1A) (AA 1-460) antibody
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Mouse
- Epitope
- AA 1-460
- Isotype
- IgG
- Vial size
- 50 μg
- Storage
- May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
- Handling
- Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Histone H3 monomethyl Lys79 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 monomethyl Lys79 antibody for monomethyl lysine 79 of histone H3. Peptides corresponding to the region around lysine 79 of histone H3 were spotted onto PVDF and probed with the antibody at a dilution of either 1:5,000 (panel A) or 1:50,000 (panel B). The amount of peptide (in picomoles) spotted is indicated next to each row. Lane 1: Unmodified Lys79 peptide. Lane 2: Monomethyl Lys79 peptide. Lane 3: Dimethyl Lys79 peptide. Lane 4: Trimethyl Lys79 peptide.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Histone H3 monomethyl Lys79 antibody tested by Western blot. HeLa acid extract (10 μg) was probed with Histone H3 monomethyl Lys79 antibody (1:1,000 dilution).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Histone H3 monomethyl Lys79 antibody (pAb) tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa chromatin (1.5 x 106 cell equivalents per ChIP) using 10 μl of Histone H3 monomethyl Lys79 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on ChIP DNA using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal compared to the negative control IgG (which has been normalized to 1.0) using the ddCT method.
