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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NBP2-29518 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal PTPN3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for ~104k PTPH1 protein phosphorylated at Ser459. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is also completely eliminated by treatment with lambda phosphatase.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: PTPN3 [p Ser459] Antibody [NBP2-29518] - Western blot of mouse testes lysate showing specific immunolabeling of the ~104k PTPH1 phosphorylated at Ser459 (Control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (400 units/100ul lysate for 30 min, RT) before being exposed to the phospho-Ser459 PTPH1 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
