Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 12321-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12321-1-AP, RRID:AB_2177510
- Product name
- RAB3IP/Rabin8 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated RAB3IP/Rabin8 antibody (Cat. #12321-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Guanine nucleotide exchange factors activate Rab8a for Toll-like receptor signalling.
Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.
The C7orf43/TRAPPC14 component links the TRAPPII complex to Rabin8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.
Akt Regulates a Rab11-Effector Switch Required for Ciliogenesis.
Golgi-resident TRIO regulates membrane trafficking during neurite outgrowth.
Cytoplasmic control of Rab family small GTPases through BAG6.
Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion.
Phosphoproteomic screening identifies Rab GTPases as novel downstream targets of PINK1.
GSK3β-Dzip1-Rab8 cascade regulates ciliogenesis after mitosis.
Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.
RAB8B is required for activity and caveolar endocytosis of LRP6.
Chemical genetic identification of NDR1/2 kinase substrates AAK1 and Rabin8 Uncovers their roles in dendrite arborization and spine development.
Rabin8 Protein Interacts with GTPase Rheb and Inhibits Phosphorylation of Ser235/Ser236 in Small Ribosomal Subunit Protein S6.
A molecular network for de novo generation of the apical surface and lumen.
Tong SJ, Wall AA, Hung Y, Luo L, Stow JL
Small GTPases 2021 Jan;12(1):27-43
Small GTPases 2021 Jan;12(1):27-43
Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.
Miyamoto T, Hosoba K, Itabashi T, Iwane AH, Akutsu SN, Ochiai H, Saito Y, Yamamoto T, Matsuura S
The EMBO journal 2020 Jun 17;39(12):e103499
The EMBO journal 2020 Jun 17;39(12):e103499
The C7orf43/TRAPPC14 component links the TRAPPII complex to Rabin8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.
Cuenca A, Insinna C, Zhao H, John P, Weiss MA, Lu Q, Walia V, Specht S, Manivannan S, Stauffer J, Peden AA, Westlake CJ
The Journal of biological chemistry 2019 Oct 18;294(42):15418-15434
The Journal of biological chemistry 2019 Oct 18;294(42):15418-15434
Akt Regulates a Rab11-Effector Switch Required for Ciliogenesis.
Walia V, Cuenca A, Vetter M, Insinna C, Perera S, Lu Q, Ritt DA, Semler E, Specht S, Stauffer J, Morrison DK, Lorentzen E, Westlake CJ
Developmental cell 2019 Jul 22;50(2):229-246.e7
Developmental cell 2019 Jul 22;50(2):229-246.e7
Golgi-resident TRIO regulates membrane trafficking during neurite outgrowth.
Tao T, Sun J, Peng Y, Li Y, Wang P, Chen X, Zhao W, Zheng YY, Wei L, Wang W, Zhou Y, Liu J, Shi YS, Zhu MS
The Journal of biological chemistry 2019 Jul 12;294(28):10954-10968
The Journal of biological chemistry 2019 Jul 12;294(28):10954-10968
Cytoplasmic control of Rab family small GTPases through BAG6.
Takahashi T, Minami S, Tsuchiya Y, Tajima K, Sakai N, Suga K, Hisanaga SI, Ohbayashi N, Fukuda M, Kawahara H
EMBO reports 2019 Apr;20(4)
EMBO reports 2019 Apr;20(4)
Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion.
Chen L, Chen Q, Xie B, Quan C, Sheng Y, Zhu S, Rong P, Zhou S, Sakamoto K, MacKintosh C, Wang HY, Chen S
Proceedings of the National Academy of Sciences of the United States of America 2016 Jun 28;113(26):7219-24
Proceedings of the National Academy of Sciences of the United States of America 2016 Jun 28;113(26):7219-24
Phosphoproteomic screening identifies Rab GTPases as novel downstream targets of PINK1.
Lai YC, Kondapalli C, Lehneck R, Procter JB, Dill BD, Woodroof HI, Gourlay R, Peggie M, Macartney TJ, Corti O, Corvol JC, Campbell DG, Itzen A, Trost M, Muqit MM
The EMBO journal 2015 Nov 12;34(22):2840-61
The EMBO journal 2015 Nov 12;34(22):2840-61
GSK3β-Dzip1-Rab8 cascade regulates ciliogenesis after mitosis.
Zhang B, Zhang T, Wang G, Wang G, Chi W, Jiang Q, Zhang C
PLoS biology 2015 Apr;13(4):e1002129
PLoS biology 2015 Apr;13(4):e1002129
Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.
Burke MC, Li FQ, Cyge B, Arashiro T, Brechbuhl HM, Chen X, Siller SS, Weiss MA, O'Connell CB, Love D, Westlake CJ, Reynolds SD, Kuriyama R, Takemaru K
The Journal of cell biology 2014 Oct 13;207(1):123-37
The Journal of cell biology 2014 Oct 13;207(1):123-37
RAB8B is required for activity and caveolar endocytosis of LRP6.
Demir K, Kirsch N, Beretta CA, Erdmann G, Ingelfinger D, Moro E, Argenton F, Carl M, Niehrs C, Boutros M
Cell reports 2013 Sep 26;4(6):1224-34
Cell reports 2013 Sep 26;4(6):1224-34
Chemical genetic identification of NDR1/2 kinase substrates AAK1 and Rabin8 Uncovers their roles in dendrite arborization and spine development.
Ultanir SK, Hertz NT, Li G, Ge WP, Burlingame AL, Pleasure SJ, Shokat KM, Jan LY, Jan YN
Neuron 2012 Mar 22;73(6):1127-42
Neuron 2012 Mar 22;73(6):1127-42
Rabin8 Protein Interacts with GTPase Rheb and Inhibits Phosphorylation of Ser235/Ser236 in Small Ribosomal Subunit Protein S6.
Parkhitko CA, Favorova CO, Henske EP
Acta naturae 2011 Jul;3(3):71-6
Acta naturae 2011 Jul;3(3):71-6
A molecular network for de novo generation of the apical surface and lumen.
Bryant DM, Datta A, Rodríguez-Fraticelli AE, Peränen J, Martín-Belmonte F, Mostov KE
Nature cell biology 2010 Nov;12(11):1035-45
Nature cell biology 2010 Nov;12(11):1035-45
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human brain tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP(RABIN8 antibody) at dilution of 1:2000
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using RAB3IP antibody 12321-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-RABIN8 (IP:12321-1-AP, 3ug; Detection:12321-1-AP 1:1000) with mouse lung tissue lysate 4500ug.
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human pancreas cancer using 12321-1-AP(RABIN8 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue