PA5-96927

antibody from Invitrogen Antibodies

Targeting: RAB3IP FLJ22548, RABIN3, RABIN8

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96927

Provider

Invitrogen Antibodies

Product name

RAB3IP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MANDPLEGFH EVNLASPTSP DLLGVYESGT QEQTTSPSVI YRPHPSALSS VPIQANALDV SELPTQPVYS SPRRLNCAEI SSISFHVTDP APCSTSGVTA GLTKLTTRKD NYNAEREFLQ GATITEACDG SDDIFGLSTD SLSRLRSPSV; Positive Samples: HeLa, A-549, 293T, Mouse heart, Mouse kidney, Mouse brain, Mouse liver, Rat brain, Rat testis; Cellular Location: Cell projection, Cytoplasm, Nucleus, cytoskeleton, lamellipodium

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.73 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

MicroRNA‑126 protects SH‑SY5Y cells from ischemia/reperfusion injury‑induced apoptosis by inhibiting RAB3IP.

Sun Z, Zhao X, Zhang M, Li N, Zhao Y, Chen C, Li J, Guo Y, Feng Q
Molecular medicine reports 2022 Feb;25(2)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RAB3IP in extracts of various cell lines using RAB3IP Polyclonal Antibody (Product # PA5-96927) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RAB3IP in paraffin-embedded mouse brain using RAB3IP Polyclonal Antibody (Product # PA5-96927) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RAB3IP in paraffin-embedded rat brain using RAB3IP Polyclonal Antibody (Product # PA5-96927) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RAB3IP in paraffin-embedded human lung cancer using RAB3IP Polyclonal Antibody (Product # PA5-96927) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RAB3IP in paraffin-embedded human stomach using RAB3IP Polyclonal Antibody (Product # PA5-96927) at a dilution of 1:200.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3. Effect of miR-126 on RAB3IP expression during cerebral ischemia/reperfusion injury. RAB3IP (A) mRNA and (B) protein expression levels were determined using reverse transcription-quantitative PCR and western blotting, respectively. n=3. The data were analyzed using one-way ANOVA followed by Tukey's post hoc test. **P

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4. miR-126 regulates cell viability and apoptosis during cerebral ischemia/reperfusion injury via RAB3IP. RAB3IP (A) mRNA and (B) protein expression levels were determined using reverse transcription-quantitative PCR and western blotting, respectively. (C) miR-126 expression levels. (D) Cell viability. (E-H) Apoptosis was determined using AV/PI and Hoechst/PI assays. n=3. The data were analyzed using one-way ANOVA followed by Tukey's post hoc test. *P