Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
- Other assay [1]
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Validation data
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- Product number
- 11369-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DEF6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MALRKELLK SIWYAFTALD VEKSGKVSKS QLKVLSHNLY TVLHIPHDPV ALEEHFRDDD DGPVSSQGYM PYLNKYILDK VEEGAFVKEH FDELCWTLTA KKNYRADSNG NSMLSNQDAF RLWCLFNFLS EDKYPLIMVP DEVEYLLKKV LSSMSLEVSL GELEELLAQE AQVAQTTGGL SVWQFLELFN SGRCLRGVGR DTLSMAIHEV YQELIQDVLK QGYLWKRGHL RRNWAERWFQ LQPSCLCYFG SEECKEKRGI IPLDAHCCVE VLPDRDGKRC MFCVKTATRT YEMSASDTRQ RQEWTAAIQM AIRLQAEGKT SLHKDLKQKR REQREQR (1-336 aa encoded by BC017504)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.2 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of DEF6 antibody (11369-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DEF6 transfected Jurkat cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 11369-1-AP ( DEF6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 11369-1-AP (DEF6 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 11369-1-AP (DEF6 Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse spleen tissue slide using 11369-1-AP ( DEF6 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse spleen tissue slide using 11369-1-AP ( DEF6 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-DEF6 (IP:11369-1-AP, 4ug; Detection:11369-1-AP 1:600) with Jurkat cells lysate 2400ug.