TA328649

antibody from OriGene

Targeting: P2RY12 HORK3, P2Y12, SP1999

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA328649

Provider

OriGene

Product name

Rabbit Polyclonal Anti-P2Y12 Receptor (extracellular)

Antibody type

Polyclonal

Description

Rabbit Polyclonal Anti-P2Y12 Receptor (extracellular)

Host

Rabbit

Conjugate

Unconjugated

Epitope

P2RY12

Antibody clone number

NULL

Vial size

160 µg

Concentration

NULL

References

Submitted references

The role of agonist-induced activation and inhibition for the regulation of purinergic receptor expression in human platelets.

Koessler J, Trulley VN, Bosch A, Weber K, Koessler A, Boeck M, Kobsar A
Thrombosis research 2018 Aug;168:40-46

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of rat brain membrane (lanes 1 and 3) and MEG-01 (lanes 2 and 4) cell line lysate: 1-2. Anti-P2Y12 Receptor (extracellular) antibody, (1:200). 3-4. Anti-P2Y12 Receptor (extracellular) antibody, preincubated with the control peptide antigen.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Expression of P2Y12 in rat RBL cells.Immunocytochemical staining of intact living rat basophilic leukemia (RBL) cells using. A. Extracellular staining of cells using Anti-P2Y12 (extracellular) antibody , (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells.

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Indirect flow cytometry analysis of MEG-O1 living cells: black line: Unstained cells. Green line: Cells + Anti-P2Y12 Receptor (extracellular) antibody, (5-10 ug/1x106 cells).

Validation comment

FC