Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF4269 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Nephrin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Nephrin in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse Nephrin is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
O60500
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Organoid single cell profiling identifies a transcriptional signature of glomerular disease.
3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening.
ELMO1 protects renal structure and ultrafiltration in kidney development and under diabetic conditions.
Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis.
Harder JL, Menon R, Otto EA, Zhou J, Eddy S, Wys NL, O'Connor C, Luo J, Nair V, Cebrian C, Spence JR, Bitzer M, Troyanskaya OG, Hodgin JB, Wiggins RC, Freedman BS, Kretzler M, European Renal cDNA Bank (ERCB), Nephrotic Syndrome Study Network (NEPTUNE)
JCI insight 2019 Jan 10;4(1)
JCI insight 2019 Jan 10;4(1)
3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening.
Hale LJ, Howden SE, Phipson B, Lonsdale A, Er PX, Ghobrial I, Hosawi S, Wilson S, Lawlor KT, Khan S, Oshlack A, Quinlan C, Lennon R, Little MH
Nature communications 2018 Dec 4;9(1):5167
Nature communications 2018 Dec 4;9(1):5167
ELMO1 protects renal structure and ultrafiltration in kidney development and under diabetic conditions.
Sharma KR, Heckler K, Stoll SJ, Hillebrands JL, Kynast K, Herpel E, Porubsky S, Elger M, Hadaschik B, Bieback K, Hammes HP, Nawroth PP, Kroll J
Scientific reports 2016 Nov 16;6:37172
Scientific reports 2016 Nov 16;6:37172
Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis.
Takasato M, Er PX, Chiu HS, Maier B, Baillie GJ, Ferguson C, Parton RG, Wolvetang EJ, Roost MS, Chuva de Sousa Lopes SM, Little MH
Nature 2015 Oct 22;526(7574):564-8
Nature 2015 Oct 22;526(7574):564-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Nephrin by Western Blot. Western blot shows lysates of human kidney tissue under reducing and non-reducing conditions. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4269) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for Nephrin was detected at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Nephrin in Human Kidney. Nephrin was detected in immersion fixed paraffin-embedded sections of human kidney using 1.7 µg/mL Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4269) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to podocytes in glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.