Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF704 - Provider product page
- Provider
- R&D Systems
- Product name
- Human AgRP/ART Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human AgRP/ART in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse AgRP/ART is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O00253
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- AgRP/ART in Human Brain. AgRP/ART was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human AgRP/ART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF704) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown ; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.