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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00065057-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00065057-M02, RRID:AB_605894
- Product name
- ACD monoclonal antibody (M02), clone 1D8-1B6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant ACD.
- Antigen sequence
MPGRCQSDAAMRVNGPASRAPAGWTSGSLHTGPRA
GRPRAQARGVRGRGLLLRPRPAKELPLPRKGGAWA
PAGNPGPLHPLGVAVGMAGSGRLVLRPWIRELILG
SETPSSPRAGQLLEVLQDAEAAVAGPSHAPDTSDV
GATLLVSDGTHSVRCLVTREALDTSDWEEKEFGFR
GTEGRLLLLQDCGVHVQVAEGGAPAEFYLQVDRFS
LLPTEQPRLRVPGCNQDLDVQKKLYDCLEEHLSES
TSSNAGLSLSQLLDEMREDQEHQGALVCLAESCLT
LEGPCTAPPVTHWAASRCKATGEAVYTVPSSMLCI
SENDQLILSSLGPCQRTQGPELPPPDPALQDLSLT
LIASPPSSPSSSGTPALPGHMSSEESGTSISLLPA
LSLAAPDPGQRSSSQPSPAICSAPATLTPRSPHAS
RTPSSPLQSCTPSLSPRSHVPSPHQALVTRPQKPS
LEFKEFVGLPCKNRPPFPRTGATRGAQEPCSVWEP
PKRHRDGSAFQYEYEPPCTSLCARVQAARLPPQLM
AWALHFLMDAQPGSEPTPM- Isotype
- IgG
- Antibody clone number
- 1D8-1B6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references The Shelterin TIN2 Subunit Mediates Recruitment of Telomerase to Telomeres.
TIN2-tethered TPP1 recruits human telomerase to telomeres in vivo.
Frank AK, Tran DC, Qu RW, Stohr BA, Segal DJ, Xu L
PLoS genetics 2015 Jul;11(7):e1005410
PLoS genetics 2015 Jul;11(7):e1005410
TIN2-tethered TPP1 recruits human telomerase to telomeres in vivo.
Abreu E, Aritonovska E, Reichenbach P, Cristofari G, Culp B, Terns RM, Lingner J, Terns MP
Molecular and cellular biology 2010 Jun;30(12):2971-82
Molecular and cellular biology 2010 Jun;30(12):2971-82
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ACD is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to ACD on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of ACD transfected lysate using anti-ACD monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ACD MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to ACD on formalin-fixed paraffin-embedded human heart tissue. [antibody concentration 2 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
