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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00065057-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00065057-M01, RRID:AB_1672234
- Product name
- ACD monoclonal antibody (M01), clone 1C11-1A7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant ACD.
- Antigen sequence
MPGRCQSDAAMRVNGPASRAPAGWTSGSLHTGPRA
GRPRAQARGVRGRGLLLRPRPAKELPLPRKGGAWA
PAGNPGPLHPLGVAVGMAGSGRLVLRPWIRELILG
SETPSSPRAGQLLEVLQDAEAAVAGPSHAPDTSDV
GATLLVSDGTHSVRCLVTREALDTSDWEEKEFGFR
GTEGRLLLLQDCGVHVQVAEGGAPAEFYLQVDRFS
LLPTEQPRLRVPGCNQDLDVQKKLYDCLEEHLSES
TSSNAGLSLSQLLDEMREDQEHQGALVCLAESCLT
LEGPCTAPPVTHWAASRCKATGEAVYTVPSSMLCI
SENDQLILSSLGPCQRTQGPELPPPDPALQDLSLT
LIASPPSSPSSSGTPALPGHMSSEESGTSISLLPA
LSLAAPDPGQRSSSQPSPAICSAPATLTPRSPHAS
RTPSSPLQSCTPSLSPRSHVPSPHQALVTRPQKPS
LEFKEFVGLPCKNRPPFPRTGATRGAQEPCSVWEP
PKRHRDGSAFQYEYEPPCTSLCARVQAARLPPQLM
AWALHFLMDAQPGSEPTPM- Isotype
- IgG
- Antibody clone number
- 1C11-1A7
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ACD expression in transfected 293T cell line by ACD monoclonal antibody (M01), clone 1C11-1A7.Lane 1: ACD transfected lysate (Predicted MW: 57.7 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ACD is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of ACD transfected lysate using anti-ACD monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ACD MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
