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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA504406 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504406, RRID:AB_11123790
- Product name
- ACD mouse monoclonal antibody, clone OTI1A2 (formerly 1A2)
- Antibody type
- Monoclonal
- Description
- ACD mouse monoclonal antibody, clone OTI1A2 (formerly 1A2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ACD
- Isotype
- IgG
- Antibody clone number
- OTI1A2
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACD (RC204381, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACD.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Western blot analysis of TPP1 (also known as ACD) protein expression by using anti-ACD antibody in NCI-N87 cells.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-ACD mouse monoclonal antibody (TA504406) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACD(RC204381).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC204381 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACD antibody(TA504406), and then analyzed by flow cytometry.
- Validation comment
- FC
