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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF3239 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Semaphorin 3E Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Semaphorin 3E in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Semaphorin 3A, rhSemaphorin 3C, rhSemaphorin 3F, and rhSemaphorin 3G is observed.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references TG-interacting factor 1 (Tgif1)-deficiency attenuates bone remodeling and blunts the anabolic response to parathyroid hormone.
Expression of semaphorin 3E is suppressed in severe asthma.
Neuroimmune guidance cue Semaphorin 3E is expressed in atherosclerotic plaques and regulates macrophage retention.
Dynamics of the skeletal muscle secretome during myoblast differentiation.
Saito H, Gasser A, Bolamperti S, Maeda M, Matthies L, Jähn K, Long CL, Schlüter H, Kwiatkowski M, Saini V, Pajevic PD, Bellido T, van Wijnen AJ, Mohammad KS, Guise TA, Taipaleenmäki H, Hesse E
Nature communications 2019 Mar 22;10(1):1354
Nature communications 2019 Mar 22;10(1):1354
Expression of semaphorin 3E is suppressed in severe asthma.
Movassagh H, Shan L, Chakir J, McConville JF, Halayko AJ, Koussih L, Gounni AS
The Journal of allergy and clinical immunology 2017 Oct;140(4):1176-1179
The Journal of allergy and clinical immunology 2017 Oct;140(4):1176-1179
Neuroimmune guidance cue Semaphorin 3E is expressed in atherosclerotic plaques and regulates macrophage retention.
Wanschel A, Seibert T, Hewing B, Ramkhelawon B, Ray TD, van Gils JM, Rayner KJ, Feig JE, O'Brien ER, Fisher EA, Moore KJ
Arteriosclerosis, thrombosis, and vascular biology 2013 May;33(5):886-93
Arteriosclerosis, thrombosis, and vascular biology 2013 May;33(5):886-93
Dynamics of the skeletal muscle secretome during myoblast differentiation.
Henningsen J, Rigbolt KT, Blagoev B, Pedersen BK, Kratchmarova I
Molecular & cellular proteomics : MCP 2010 Nov;9(11):2482-96
Molecular & cellular proteomics : MCP 2010 Nov;9(11):2482-96
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Semaphorin 3E in Mouse Brain. Semaphorin 3E was detected in immersion fixed frozen sections of adult mouse brain using Goat Anti-Human Semaphorin 3E Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3239) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cerebellum. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
