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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-1433 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1433, RRID:AB_2185551
- Product name
- Goat Polyclonal Semaphorin 3E Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Semaphorin 3E and plexin-D1 control vascular pattern independently of neuropilins.
Gu C, Yoshida Y, Livet J, Reimert DV, Mann F, Merte J, Henderson CE, Jessell TM, Kolodkin AL, Ginty DD
Science (New York, N.Y.) 2005 Jan 14;307(5707):265-8
Science (New York, N.Y.) 2005 Jan 14;307(5707):265-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Semaphorin 3E Antibody [NB100-1433] - Staining (0.1ug/ml) of Human Breast lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Semaphorin 3E Antibody [NB100-1433] - Human Adrenal Gland at 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
