PAB3198

antibody from Abnova Corporation

Targeting: MAP4K3 GLK, MAPKKKK3, RAB8IPL1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB3198

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB3198, RRID:AB_1577194

Product name

MAP4K3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of MAP4K3.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Activation of the c-Jun N-terminal kinase pathway by a novel protein kinase related to human germinal center kinase.

Diener K, Wang XS, Chen C, Meyer CF, Keesler G, Zukowski M, Tan TH, Yao Z
Proceedings of the National Academy of Sciences of the United States of America 1997 Sep 2;94(18):9687-92

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of MAP4K3 polyclonal antibody (Cat # PAB3198) in HeLa cell lysate. Dilution for MAP4K3 polyclonal antibody (Cat # PAB3198) was 1 : 100 ; dilution for secondary goat anti-rabbit-HRP was 1 : 7000. A chemiluminescent kit was used for development of the Western blot. Data and protocol courtesy of Dr. Richard Lu, Partners HealthCare System at Harvard University.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAP4K3 polyclonal antibody (Cat # PAB3198) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)