PA5-30485

antibody from Invitrogen Antibodies

Targeting: EDC4 Ge-1, HEDLS, RCD-8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30485

Provider

Invitrogen Antibodies

Product name

EDC4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7, mouse brain.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using EDC4 Polyclonal Antibody (Product # PA5-30485). Sample (30 µg of whole cell lysate). Lane A: MCF-7. 5% SDS PAGE. EDC4 Polyclonal Antibody (Product # PA5-30485) diluted at 1:3,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using EDC4 Polyclonal Antibody (Product # PA5-30485). Sample (50 µg of whole cell lysate). Lane A: mouse brain . 5% SDS PAGE. EDC4 Polyclonal Antibody (Product # PA5-30485) diluted at 1:2,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of EDC4 (green) in Hela cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 2% BSA in PBS for 60 minutes at room temperature. Cells were stained with a EDC4 polyclonal antibody (Product # PA5-30485) at a dilution of 2 µg/mL in staining buffer for 3 hour at room temperature, and then incubated with a Goat anti-rabbit IgG Secondary Antibody, Alexa Fluor 488 conjugate (Product # A32731) at a dilution of 1:1000 for 1 hour at room temperature (green). Nuclei (blue) were counterstained with DAPI dye. Images were taken at 100X magnification. Data courtesy of Thermo Scientific KOL Program.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

EDC4 Polyclonal Antibody detects EDC4 protein at P-body by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EDC4 protein stained by EDC4 Polyclonal Antibody (Product # PA5-30485) diluted at 1:500. Blue: Hoechst 33343 staining. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope].

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

EDC4 Polyclonal Antibody detects EDC4 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. EDC4 Polyclonal Antibody (Product # PA5-30485) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using EDC4 (Product # PA5-30485) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.