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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00005699-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005699-M01, RRID:AB_913814
- Product name
- PSMB10 monoclonal antibody (M01), clone 3F8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant PSMB10.
- Antigen sequence
MLKPALEPRGGFSFENCQRNASLERVLPGLKVPHA
RKTGTTIAGLVFQDGVILGADTRATNDSVVADKSC
EKIHFIAPKIYCCGAGVAADAEMTTRMVASKMELH
ALSTGREPRVATVTRILRQTLFRYQGHVGASLIVG
GVDLTGPQLYGVHPHGSYSRLPFTALGSGQDAALA
VLEDRFQPNMTLEAAQGLLVEAVTAGILGDLGSGG
NVDACVITKTGAKLLRTLSSPTEPVKRSGRYHFVP
GTTAVLTQTVKPLTLELVEETVQAMEVE- Isotype
- IgG
- Antibody clone number
- 3F8
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Endoplasmic reticulum stress activates autophagy but not the proteasome in neuronal cells: implications for Alzheimer's disease.
Nijholt DA, de Graaf TR, van Haastert ES, Oliveira AO, Berkers CR, Zwart R, Ovaa H, Baas F, Hoozemans JJ, Scheper W
Cell death and differentiation 2011 Jun;18(6):1071-81
Cell death and differentiation 2011 Jun;18(6):1071-81
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PSMB10 monoclonal antibody (M01), clone 3F8 Western Blot analysis of PSMB10 expression in Jurkat ( Cat # L017V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PSMB10 is approximately 3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to PSMB10 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
