13571-1-AP

antibody from Invitrogen Antibodies

Targeting: ARFGAP1 ARF1GAP, bA261N11.3, FLJ10767

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

13571-1-AP

Provider

Invitrogen Antibodies

Product name

ARFGAP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MASPRTRKV LKEVRVQDEN NVCFECGAFN PQWVSVTYGI WICLECSGRH RGLGVHLSFV RSVTMDKWKD IELEKMKAGG NAKFREFLES QEDYDPCWSL QEKYNSRAAA LFRDKVVALA EGREWSLESS PAQNWTPPQP RTLPSMVHRV SGQPQSVTAS SDKAFEDWLN DDLGSYQGAQ GNRYVGFGNT PPPQKKEDDF LNNAMSSLYS GWSSFTTGAS RFASAAKEGA TKFGSQASQK FWGHKQQPEP ASELGHSLNE NVLKPAQEKV KEGKIFDDVS SGVSQLASKG VGSKGWRDVT TFFSGKAEGP LDSPSEGHSY QNSGLDHFQN SNIDQSFWET FGSAEPTKTR KSPSSDSWTC ADTSTERRSS DSWEVWGSAS TNRNSNSDGG EGGEGTKKAV PPAVPTDDGW DNQNW (1-414 aa encoded by BC028233)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.41 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 13571-1-AP (ARFGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 13571-1-AP (ARFGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 13571-1-AP (ARFGAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed PC-3 cells using 13571-1-AP (ARFGAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using (Product # 13571-1-AP) (ARFGAP1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed PC-3 cells using 13571-1-AP (ARFGAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer using 13571-1-AP (ARFGAP1 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer using 13571-1-AP (ARFGAP1 antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ARFGAP1 (IP:13571-1-AP, 4ug; Detection:13571-1-AP 1:500) with mouse testis tissue lysate 4000ug.