21499-1-AP
antibody from Invitrogen Antibodies
Targeting: SH3D19
DKFZp434D0215, EBP, Eve-1, EVE1, Kryn, SH3P19
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [4]
- Immunohistochemistry [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 21499-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SH3D19 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: VLVMLKQTE NNYLECQKGE DTGRVHLSQM KIITPLDEHL RSRPNDPSHA QKPVDSGAPH AVVLHDFPAE QVDDLNLTSG EIVYLLEKID TDWYRGNCRN QIGIFPANYV KVIIDIPEGG NGKRECVSSH CVKGSRCVAR FEYIGEQKDE LSFSEGEIII LKEYVNEEWA RGEVRGRTGI FPLNFVEPVE DYPTSGANVL STKVPLKTKK EDSGSNSQVN SLPAEWCEAL HSFTAETSDD LSFKRGDRIQ ILERLDSDWC RGRLQDREGI FPAVFVRPCP AEAKSMLAIV PKGRKAKALY DFRGENEDEL SFKAGDIITE LESVDDDWMS GELMGKSGIF PKNYIQFLQI S (411-790 aa encoded by BC108890)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HT-1080 cells were subjected to SDS PAGE followed by western blot with 21499-1-AP (SH3D19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Y79 cells were subjected to SDS PAGE followed by western blot with 21499-1-AP (SH3D19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using SH3D19 antibody 21499-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using SH3D19 antibody 21499-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using SH3D19 antibody 21499-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using SH3D19 antibody 21499-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 40x lens).