LS-C348746

antibody from LSBio

Targeting: E2F7

Western blot (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Gel shift (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C348746

Provider

LSBio

Product name

Anti-E2F7 Antibody LS-C348746

Antibody type

Polyclonal

Description

Antiserum

Reactivity

Human

Host

Rabbit

Vial size

100µl

Storage

Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

The antibody immunoprecipitates endogenous E2F7-A. 500 ug of whole cell extracts from K562 cells were immunoprecipitated with 2 ul of the crude rabbit serum or the corresponding pre-immune serum (PI). Immunoprecipitated proteins were fractionated on 8% SDSPAGE and transferred to nitrocellulose membranes. The membranes were then probed with a previously described anti-E2F7 antibody (1). Peroxidase-conjugated anti-rabbit antibodies followed by ECL were then used. The arrow points to immunoprecipitated E2F7-A.

Gel shift

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

The anti-E2F7-A antibody is able to supershift a DNA-protein complex that contains E2F7-A. Complex formation employing a radiolabeled E2F element from the human DHFR promoter and nuclear extracts from mock transfected Saos-2 cells (-) or nuclear extracts from Saos-2 cells transfected with pcDNA3-HAE2F7- A (+) were analyzed by EMSA. Extracts were preincubated in the absence (None) or in the presence of an antibody to the HA tag or the anti-E2F7-A antibody. The arrow indicates the position of the HA-E2F7-A containing complex, whereas arrowheads indicate the position of supershifted complexes.