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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [2]
- Immunohistochemistry [1]
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- Product number
- H00083667-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00083667-M03, RRID:AB_2185485
- Product name
- Mouse Monoclonal SESN2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SESN2 - sestrin 2
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Arsenic trioxide-mediated suppression of miR-182-5p is associated with potent anti-oxidant effects through up-regulation of SESN2.
The inhibition of autophagy sensitises colon cancer cells with wild-type p53 but not mutant p53 to topotecan treatment.
Association of platelet-derived growth factor receptor β accumulation with increased oxidative stress and cellular injury in sestrin 2 silenced human glioblastoma cells.
Lin LT, Liu SY, Leu JD, Chang CY, Chiou SH, Lee TC, Lee YJ
Oncotarget 2018 Mar 23;9(22):16028-16042
Oncotarget 2018 Mar 23;9(22):16028-16042
The inhibition of autophagy sensitises colon cancer cells with wild-type p53 but not mutant p53 to topotecan treatment.
Li DD, Sun T, Wu XQ, Chen SP, Deng R, Jiang S, Feng GK, Pan JX, Zhang XS, Zeng YX, Zhu XF
PloS one 2012;7(9):e45058
PloS one 2012;7(9):e45058
Association of platelet-derived growth factor receptor β accumulation with increased oxidative stress and cellular injury in sestrin 2 silenced human glioblastoma cells.
Liu SY, Lee YJ, Lee TC
FEBS letters 2011 Jun 23;585(12):1853-8
FEBS letters 2011 Jun 23;585(12):1853-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SESN2 Antibody (3B8) [H00083667-M03] - SESN2 expression in K-562 ( Cat # L009V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SESN2 Antibody (3B8) [H00083667-M03] - Analysis of SESN2 expression in transfected 293T cell line by SESN2 monoclonal antibody (M03), clone 3B8. Lane 1: SESN2 transfected lysatE (54.5 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: SESN2 Antibody (3B8) [H00083667-M03] - Detection limit for recombinant GST tagged SESN2 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: SESN2 Antibody (3B8) [H00083667-M03] - Analysis of monoclonal antibody to SES2 on HeLa cell. Antibody concentration 10 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SESN2 Antibody (3B8) [H00083667-M03] - SESN2 transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SESN2 Antibody (3B8) [H00083667-M03] - Analysis of SESN2 transfected lysate using anti-SESN2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SESN2 rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SESN2 Antibody (3B8) [H00083667-M03] - Analysis of monoclonal antibody to SESN2 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml
