10795-1-AP

antibody from Invitrogen Antibodies

Targeting: SESN2 DKFZp761M0212, HI95, SES2, SEST2

Provider product page for 10795-1-AP

Western blot

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [0]
Comments [0]
Validations
Western blot [13]
Immunocytochemistry [1]
Immunohistochemistry [11]
Other assay [1]

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Product number: 10795-1-AP - Provider product page
Provider: Invitrogen Antibodies
Product name: Sestrin 2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Description: Immunogen sequence: HMAEFLQTG GDPEWLLGLH RAPEKLRKLS EINKLLAHRP WLITKEHIQA LLKTGEHTWS LAELIQALVL LTHCHSLSSF VFGCGILPEG DADGSPAPQA PTPPSEQSSP PSRDPLNNSG GFESARDVEA LMERMQQLQE SLLRDEGTSQ EEMESRFELE KSESLLVTPS ADILEPSPHP DMLCFVEDPT FGYEDFTRRG AQAPPTFRAQ DYTWEDHGYS LIQRLYPEGG QLLDEKFQAA YSLTYNTIAM HSGVDTSVLR RAIWNYIHCV FGIRYDDYDY GEVNQLLERN LKVYIKTVAC YPEKTTRRMY NLFWRHFRHS EKVHVNLLLL EARMQAALLY ALRAITRYMT (132-480 aa encoded by BC013304)
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 150 µL
Concentration: 0.48 mg/mL
Storage: -20°C

SESN2 protein structure - 10795-1-AP shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Various lysates were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: WB result of Sestrin antibody (10795-1-AP, 1:2000) with si-Control and si-Sestrin transfected HEK293 cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Rat liver tissue were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody at dilution of 1:1000 incubated at 4 degree celsius over night.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: A549 cells (shcontrol and shRNA of SESN2) were subjected to SDS PAGE followed by western blot with 10795-1-AP (SESN2 antibody) at dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: A2780 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Human liver tissue were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Human brain tissue were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Various lysates were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human ovary tumor using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human ovary tumor using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human breast cancer using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human breast cancer using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human ovary tumor using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:50 (under 25x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10795-1-AP (Sestrin 2 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10795-1-AP (Sestrin 2 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).