Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- ANC-004-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain cell lysates: - 1,2. Anti-Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Antibody (#ANC-004), (1:200).3,4. Anti-Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Antibody , preincubated with Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 (extracellular) Blocking Peptide (#BLP-NC004).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain cell lysates: - 1,2. Anti-Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Antibody (#ANC-004), (1:200).3,4. Anti-Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Antibody , preincubated with Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 (extracellular) Blocking Peptide (#BLP-NC004).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Multiplex staining of nAChR alpha 4 and nAChR beta 2 in mouse brain - Immunohistochemical staining of perfusion-fixed frozen mouse parietal cortex sections using Anti-Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Antibody (#ANC-004), (1:300) and Anti-Nicotinic Acetylcholine Receptor beta 2 (CHRNB2) (extracellular)-ATTO Fluor-594 Antibody (#ANC-012-AR), (1:60).A. nAChR alpha 4 staining (green). B. Same section stained for nAChR beta 2 (red). C. Merge of the two images reveals several cells expressing both receptors (arrows). Nuclei are stained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of nAChR alpha 4 in rat cerebellum - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Antibody (#ANC-004), (1:200). A. Expression of nAChR alpha 4 (green) was visualized in rat cerebellum. Purkinje cells were positive for nAChR alpha 4 (arrows). B. The same section was stained for Parvalbumin (red) and counterstained (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection ofnAChR alpha 4by indirect flow cytometry in live intacthumanJurkatT-cell leukemia cells: - (black line) cells. (red) Cells+ goat- Anti-rabbit-FITC. (green) Cells + Anti-Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Antibody (#ANC-004), (2.5μg) + goat- Anti-rabbit-FITC.